Question 1

Transformation and transfection are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 2

The various techniques by which scientists manipulate DNA in the lab are called

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) A) and D)
G) B) and E)

Question 3

The primers in PCR are

Accepted Answer

A)  synthetic DNA oligonucleotides.
B)  bacterial enzymes.
C)  short RNA strands.
D)  DNA polymerases.
E)  reverse transcriptases.F) C) and D)
G) B) and D)

Question 4

Which PCR step synthesizes complementary DNA strands?

Accepted Answer

A)  add DNA polymerase and nucleotides at 72 ∘\circ∘ C
B)  cool DNA to between 50 ∘\circ∘ C and 65 ∘\circ∘ C
C)  add primers
D)  heat target DNA to 94 ∘\circ∘ C
E)  repeat the cycle of heating and cooling F) A) and E)
G) B) and C)

Question 5

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Accepted Answer

A)  used as cloning vectors.
B)  sources of heat stable DNA polymerases.
C)  genetically engineered bacteria.
D)  principal sources of restriction endonucleases.
E)  None of the choices are correct.F) A) and D)
G) A) and B)

Question 6

All of the following are desirable features in a microbial cloning host, except it

Accepted Answer

A)  can be grown in large quantities.
B)  has a slow growth rate.
C)  is capable of accepting plasmid vectors.
D)  will secrete a high yield of proteins from expressed foreign genes.
E)  has a genome that is well delineated.Learning Outcome: 10.06 Characterize the events in cloning, using an example.Topic: Basics of Genetic EngineeringF) C) and D)
G) A) and B)

Question 7

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

The answer of The size of DNA is often given...

Question 8

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Accepted Answer

A)  RNA promoters
B)  fluorescent nucleotides
C)  primers
D)  thermophilic polymerases
E)  endonucleasesF) B) and E)
G) A) and D)

Question 9

A technique that separates a readable pattern of DNA fragments is

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) A) and B)
G) A) and C)

Question 10

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Accepted Answer

The answer of In recombinant DNA technique, an enzyme called...

Question 11

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Accepted Answer

A DNA fingerprint is a unique pattern of...

Question 12

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Accepted Answer

The answer of Restriction endonucleases recognize and clip at DNA...

Question 13

Which step involves transformation?

Accepted Answer

A)  target DNA removed from cells and isolated
B)  cloning host takes up a plasmid
C)  target DNA and plasmid treated with the same restriction endonuclease
D)  desired protein is produced by cloning host
E)  gene is amplified by multiplication of cloning hostF) B) and D)
G) B) and C)

Question 14

The process of introducing a needed, normal gene, into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 15

The use of an organism's biochemical processes to create a product is

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) A) and D)
G) A) and C)

Question 16

DNA polymerases used in PCR

Accepted Answer

A)  use an RNA template to make complementary DNA.
B)  must remain active at very cold temperatures.
C)  include Taq polymerases and Vent polymerase.
D)  are labeled with fluorescent dyes.
E)  All of the choices are correct.F) A) and E)
G) A) and D)

Question 17

Transgenic animals

Accepted Answer

A)  can be engineered to become factories for manufacturing proteins.
B)  are often obtained from germ line engineering.
C)  will pass the genes on to their offspring.
D)  commonly include mice.
E)  All of the choices are correct.F) A) and E)
G) C) and D)

Question 18

The commercial product Frostban contains a genetically engineered strain of

Accepted Answer

A)  Escherichia coli.
B)  Saccharomyces cerevisiae.
C)  Thermus aquaticus.
D)  Pseudomonas syringae.
E)  Pseudomonas fluorescens.F) A) and C)
G) B) and D)

Question 19

The Southern Blot technique detects

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) A) and B)
G) B) and D)

Question 20

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

The primers in PCR are

Correct AnswerverifiedShow Answer

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Correct AnswerverifiedShow Answer

The process of introducing a needed, normal gene, into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

The use of an organism's biochemical processes to create a product is

Correct AnswerverifiedShow Answer

The Southern Blot technique detects

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DNA polymerases used in PCR

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complementary DNA strands?

Correct AnswerverifiedShow Answer

Restriction endonucleases recognize and clip at DNA base sequences called _____.

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Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Correct AnswerverifiedShow Answer

Transgenic animals

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The size of DNA is often given in the number of _____ that it contains.

Correct AnswerverifiedShow Answer

All of the following are desirable features in a microbial cloning host, except it

Correct AnswerverifiedShow Answer

Transformation and transfection are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are called

Correct AnswerverifiedShow Answer

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Correct AnswerAnswered by ExamLex AIA DNA fingerprint is a unique pattern of...View AnswerShow Answer

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Correct AnswerverifiedShow Answer

Which step involves transformation?

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A technique that separates a readable pattern of DNA fragments is

Correct AnswerverifiedShow Answer

The commercial product Frostban contains a genetically engineered strain of

Correct AnswerverifiedShow Answer

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A DNA fingerprint is a unique pattern of...
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