Question 1

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 2

Transgenic animals are _____ modified organisms.

Accepted Answer

The answer of Transgenic animals are _____ modified organisms....

Question 3

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Accepted Answer

A)  PCR
B)  genetic engineering
C)  antisense technology
D)  microarray analysis
E)  DNA sequencingF) B) and E)
G) C) and D)

Question 4

When trying to locate a mutation responsible for a disease, exome sequencing would provide an effective shortcut because

Accepted Answer

A)  it targets just protein-encoding sequences.
B)  it targets the sequencing or rRNA and tRNA encoding sequences of DNA.
C)  it provides the entire genome sequence.
D)  it targets the sequencing of mitochondrial DNA.E) A) and D)
F) None of the above

Question 5

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 6

The commercial product Frostban contains a genetically engineered strain of

Accepted Answer

A)  Escherichia coli.
B)  Saccharomyces cerevisiae.
C)  Thermus aquaticus.
D)  Pseudomonas syringae.
E)  Pseudomonas fluorescens.F) B) and D)
G) C) and D)

Question 7

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following provides the specificity of the CRISPR/Cas gene editing system?

Accepted Answer

A)  the amino acids at the amino terminus of the Cas nuclease
B)  the guide RNA (crRN
A)   carried by the Cas nuclease
C)  the amino acids at the carboxyl terminus of the Cas nuclease
D)  an antibody-like region of the Cas nucleaseE) A) and B)
F) A) and D)

Question 9

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Accepted Answer

The answer of The two common vectors used to transfer...

Question 10

Two different nucleic acids can _____ by uniting at their complementary sites.

Accepted Answer

The answer of Two different nucleic acids can _____ by...

Question 11

Although originally discovered in bacteria as a defense against bacteriophage infection, the CRISPR/Cas gene editing system can be used to edit the genome of any cell, whether microbial, plant, fungal, or animal.

Accepted Answer

A) True 
 B)False

Question 12

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 13

The various techniques by which scientists manipulate DNA in the lab are called

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) A) and B)
G) A) and C)

Question 14

Genomic _____ are collections of isolated genes maintained in a cloning host.

Accepted Answer

The answer of Genomic _____ are collections of isolated genes...

Question 15

One of the first uses of DNA fingerprinting was in _________ medicine.

Accepted Answer

The answer of One of the first uses of DNA...

Question 16

The primers in PCR are

Accepted Answer

A)  synthetic DNA oligonucleotides.
B)  bacterial enzymes.
C)  short RNA strands.
D)  DNA polymerases.
E)  reverse transcriptases.F) B) and C)
G) A) and C)

Question 17

Transgenic animals

Accepted Answer

A)  can be engineered to become factories for manufacturing proteins.
B)  are often obtained from germ line engineering.
C)  will pass the genes on to their offspring.
D)  commonly include mice.
E)  All of these choices are correct.F) None of the above
G) A) and B)

Question 18

The fluorescent in situ hybridization (FISH)  probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) All of the above
G) B) and C)

Question 19

Transformation and transfection are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 20

The enzyme___ adds nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated.
A.  DNA polymerase III, 3’→	5’
B.  DNA polymerase I, 3’→	5’
C.  DNA polymerase III, 5’→	3’		
D.  DNA polymerase I, 5’→	3’

Accepted Answer

The answer of The enzyme___ adds nucleotides of DNA in...

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

Genomic _____ are collections of isolated genes maintained in a cloning host.

Correct AnswerverifiedShow Answer

Although originally discovered in bacteria as a defense against bacteriophage infection, the CRISPR/Cas gene editing system can be used to edit the genome of any cell, whether microbial, plant, fungal, or animal.

Correct AnswerverifiedShow Answer

Transgenic animals

Correct AnswerverifiedShow Answer

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

One of the first uses of DNA fingerprinting was in _________ medicine.

Correct AnswerverifiedShow Answer

When DNA is heated, the two strands will separate.

Correct AnswerverifiedShow Answer

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Correct AnswerverifiedShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

Which of the following provides the specificity of the CRISPR/Cas gene editing system?

Correct AnswerverifiedShow Answer

The enzyme___ adds nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated. A. DNA polymerase III, 3’→ 5’ B. DNA polymerase I, 3’→ 5’ C. DNA polymerase III, 5’→ 3’ D. DNA polymerase I, 5’→ 3’

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are called

Correct AnswerverifiedShow Answer

Transgenic animals are _____ modified organisms.

Correct AnswerverifiedShow Answer

Two different nucleic acids can _____ by uniting at their complementary sites.

Correct AnswerverifiedShow Answer

The primers in PCR are

Correct AnswerverifiedShow Answer

The commercial product Frostban contains a genetically engineered strain of

Correct AnswerverifiedShow Answer

The fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Correct AnswerverifiedShow Answer

Transformation and transfection are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Correct AnswerverifiedShow Answer

When trying to locate a mutation responsible for a disease, exome sequencing would provide an effective shortcut because

Correct AnswerverifiedShow Answer

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The two common vectors used to transfer a piece of DNA into a cloning host are _ and _.

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The enzyme_ adds nucleotides of DNA in the _direction when DNA is being semi-conservatively replicated. A. DNA polymerase III, 3’→ 5’ B. DNA polymerase I, 3’→ 5’ C. DNA polymerase III, 5’→ 3’ D. DNA polymerase I, 5’→ 3’

Correct Answer
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