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Laboratory strains of the model organism Escherichia coli that are resistant to antibiotics are very often used in research laboratories as well as in the biotechnology industry. If cultures of such bacteria were allowed to contaminate the environment uncontrollably, it is possible that at some point, pathogenic bacteria such as Neisseria meningitidis (which causes meningitis and can cause death, especially in children) could acquire the same antibiotic-resistance gene, causing a meningitis outbreak that is difficult to treat. In this scenario, which of the following mechanisms is a more likely source of the antibiotic-resistance gene in N. meningitidis?


A) Random new gene generation
B) Intragenic mutation
C) Gene duplication
D) DNA segment shuffling
E) Horizontal gene transfer

F) A) and B)
G) A) and C)

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Which of the following is NOT true regarding the tree of life?


A) Most bacteria and archaea have 1000 to 6000 genes in their genomes.
B) Eukaryotes are more similar to archaea than to bacteria with respect to the proteins that act on their DNA.
C) Most bacteria and archaea have genome sizes between one and ten million nucleotide pairs, whereas eukaryotic genomes can be millions of times larger.
D) Archaeal species were thought to belong to the eukaryotic world before sequence analysis placed them in a separate domain of life.
E) Photosynthetic bacteria are thought to be the ancestors of the eukaryotic chloroplasts.

F) None of the above
G) B) and D)

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Scientists discover more than ten thousand new species of living organisms every year. What is shared between all of these organisms?


A) They are made of cells, whose nuclei enclose their DNA.
B) They obtain their energy from sunlight.
C) They produce and use adenosine triphosphate (ATP) .
D) Their genome contains at least 1000 genes.
E) All of the above.

F) D) and E)
G) A) and E)

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Indicate if each of the following statements is true (T) or false (F). Your answer would be a five-letter string composed of letters T and F only, e.g. FFTFF. ( ) Animals ultimately depend on bacteria for fixation of the atmospheric nitrogen. ( ) If one finds animals in an isolated ecosystem, there should be photosynthetic organisms in that ecosystem as well. ( ) Carbon fixation can be carried out by bacteria only. ( ) All eukaryotes are organotrophs. ( ) Compared to eukaryotic cells, prokaryotes show greater biochemical diversity.

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We rely on plants as our source of org...

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All cells in a multicellular organism have normally developed from a single cell and share the same genome, but can nevertheless be wildly different in their shape and function. What in the eukaryotic genome is responsible for this cell-type diversity?


A) The genes that encode transcription regulatory proteins
B) The regulatory sequences that control the expression of genes
C) The genes that code for molecules involved in receiving cellular signals
D) The genes that code for molecules involved in sending cellular signals to other cells
E) All of the above

F) C) and D)
G) All of the above

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Indicate if each of the following descriptions matches messenger RNAs (M), ribosomal RNAs (R), or transfer RNAs (T). Your answer would be a four-letter string composed of letters M, R, and T only, e.g. RRRT. ( ) They contain codons. ( ) They contain anticodons. ( ) They are (covalently) attached to amino acids. ( ) They are at the core of a complex that carries out protein synthesis.

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M T T R

Which of the following processes that happens inside a cell DOES NOT normally require consumption of free energy by the cell?


A) Replication of the genetic material
B) Import of nutrients from the environment
C) Diffusion of small molecules within the cell
D) Regulation of gene expression
E) Synthesis of enzymes that catalyze cellular reactions

F) B) and E)
G) B) and D)

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Indicate if each of the following descriptions matches lithotrophic (L), organotrophic (O), or phototrophic (P) organisms. Your answer would be a four-letter string composed of letters L, O, and P only, e.g. LLPP. ( ) They feed on other living organisms or their organic products. ( ) They are responsible for the current oxygen-rich atmosphere of the Earth. ( ) They are all known to be prokaryotic. ( ) They are the primary energy converters in hydrothermal vents in the ocean floor.

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While organotrophs (such as animals) f...

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It is a model organism used to study various eukaryotic cell and developmental processes such as cell division and cell death. Its hermaphrodite adult is composed of exactly 959 somatic (non-germ) cells, the lineage of each of which has been worked out with great precision. It is approximately 1 mm long. Which of the following describes this organism?


A) It is a vertebrate.
B) It is a plant pathogen that destroys many crops.
C) Its genome codes for a few thousand genes.
D) It can fly.
E) It can be frozen indefinitely in a state of suspended animation.

F) C) and D)
G) B) and C)

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Indicate if each of the following descriptions matches RNA (R) or DNA (D). Your answer would be a five-letter string composed of letters R and D only, e.g. RDDRR. ( ) It is mainly found as a long, double-stranded molecule. ( ) It contains the sugar ribose. ( ) It normally contains the bases thymine, cytosine, adenine, and guanine. ( ) It can normally adopt distinctive folded shapes. ( ) It can be used as the template for protein synthesis.

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Unlike DNA, cellular RNA is mainly pol...

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Which of the following groups of living organisms has the highest variation in haploid genome size?


A) Mammals
B) Fish
C) Fungi
D) Protozoa
E) Prokaryotes

F) All of the above
G) A) and B)

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In the following paragraph, fill in the blanks (indicated by numbers) with either Archaea (A), Bacteria (B), or Eukaryotes (E). Your answer would be a three-letter string composed of letters A, B, and E only, e.g. ABE. "Methanococcus jannaschii is an anaerobic thermophilic microbe that belongs to the domain ... (1) and is found in extreme environments such as hydrothermal vents. It lives at temperatures near the boiling point of water and at pressures over 200 times higher than at sea level. Its genome sequence, identified in 1996, revealed that most of its metabolic processes are similar to those in ... (2), while its genetic machinery is more similar to that of ... (3)."

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Archaea usually live in extr...

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Which of the following would you NOT expect to find in a bacterial cell?


A) Swimming using flagella
B) Having a cell wall around the plasma membrane
C) ATP production in mitochondria
D) Protein production on the ribosome
E) Sexual exchange of DNA with other bacteria

F) A) and E)
G) B) and C)

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A mutation in the cdc28 gene in the budding yeast Saccharomyces cerevisiae causes cell-cycle arrest, giving rise to unbudded cells that look like "dumbbells." Treatment of wild-type cells with nocodazole, a drug that destabilizes some cytoskeletal polymers, leads to a similar phenotype. Based only on these observations, which statement is true regarding cdc28?


A) cdc28 codes for a master regulatory kinase that phosphorylates other proteins.
B) Nocodazole binds to the protein coded by the cdc28 gene.
C) The product of the cdc28 gene is responsible for resistance to nocodazole.
D) The product of the cdc28 gene is involved in cell cycle regulation.
E) The product of cdc28 destabilizes the same cytoskeletal polymers that nocodazole also destabilizes.

F) B) and C)
G) A) and E)

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A virus …


A) is a type of cell.
B) has genetic material made of proteins.
C) can only infect a single host species.
D) can act as a vector for gene transfer.
E) cannot persist in its host for more than one cell generation.

F) C) and D)
G) A) and C)

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Out of nearly 5000 protein-coding gene families, there is a set of nearly 300 conserved gene families that are found in species from all domains of life. When one looks at the general functions assigned to these gene families, it is found that …


A) the majority of them function in cell-to-cell signaling.
B) the majority of them are poorly characterized.
C) more than one-third of them are involved in translation or amino acid transport and metabolism.
D) more than one-half of the shared families are involved in DNA replication and transcription.
E) Nearly all of them are involved in energy production and carbohydrate metabolism.

F) None of the above
G) C) and D)

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C

Mitochondria and chloroplasts are thought to have evolved from free-living aerobic bacteria that were engulfed by an ancestral anaerobic cell and established a mutually beneficial (symbiotic) relationship with it. Which of the following statements is NOT true about these organelles?


A) They are similar in size to small bacteria.
B) They have their own circular genomic DNA.
C) They have their own ribosomes.
D) They have their own transfer RNAs.
E) They are found in all eukaryotes.

F) None of the above
G) A) and D)

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It is a model organism used to study various cell processes such as regulation of the eukaryotic cell cycle. Mutants are available for every gene in its exceptionally small genome. It can live indefinitely in either a haploid or a diploid state. Which of the following describes this organism?


A) It can reproduce only asexually.
B) It is a fungus.
C) It lacks a cell wall.
D) Its cell cycle is typically much slower than that of human cells.
E) All of the above

F) C) and D)
G) A) and B)

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To trace family relationships between distantly related organisms such as humans, algae, bacteria, and archaea, one should compare their genomes in regions …


A) that evolve rapidly.
B) that have a higher mutation rate.
C) that code for proteins.
D) where mutations are hardly tolerated.
E) where most mutations are selectively neutral.

F) D) and E)
G) B) and D)

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Based on the variation of genome size and gene number in the organisms presented in the following graph, which organism has the highest number of genes per unit length of their genome? (Note the logarithmic scale.) Based on the variation of genome size and gene number in the organisms presented in the following graph, which organism has the highest number of genes per unit length of their genome? (Note the logarithmic scale.)    A)  H. sapiens B)  M. musculus C)  A. thaliana D)  C. elegans E)  E. coli


A) H. sapiens
B) M. musculus
C) A. thaliana
D) C. elegans
E) E. coli

F) C) and D)
G) A) and E)

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E

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